Endo-polygalacturonase in Saccharomyces wine yeasts: Effect of carbon source on enzyme production

16Citations
Citations of this article
29Readers
Mendeley users who have this article in their library.

Abstract

Eight wine yeast strains of Saccharomyces sp. were tested for polygalacturonase (PGase) activity, after cultivation on various carbon sources. No strain showed any activity when grown on glucose, while five strains produced PGase in the presence of galactose and polygalacturonate. These data suggest that the PGase of wine strains is repressed by glucose and induced by galactose and polygalacturonate. The existence of the PGase gene in the wine strains and its similarity with that of the laboratory strains was proved by Southern hybridization and PCR amplification. The promoter region of the PGase gene in the wine strains was slightly different from that of the laboratory strains. This possibly explains the different pattern of gene expression in wine and laboratory strains. The PGase of wine strains produced di- or tri-galacturonic acid from polygalacturonic acid, different from the fungal PGase. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Cite

CITATION STYLE

APA

Radoi, F., Kishida, M., & Kawasaki, H. (2005). Endo-polygalacturonase in Saccharomyces wine yeasts: Effect of carbon source on enzyme production. In FEMS Yeast Research (Vol. 5, pp. 663–668). Elsevier. https://doi.org/10.1016/j.femsyr.2004.09.006

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free