The activity of cellulase Enzymes of Ganoderma applanatum and Ganoderma tropicum

Abstract

Ganoderma can live as saprobes on rotting stumps and roots by decomposing lignin as well as cellulose and related polysaccharides. It is because of mushrooms cellulolytic activities. Objective: The aim of this study was to compare the cellulolytic activity of the crude extract of Ganoderma applanatum and Ganoderma tropicum from Garut (Indonesia). Method: Colorimetric method using dinitrosalicylic acid (DNS). Results: The cellulolytic activity of G. applanatum and G. tropicum in the pH range of 3.5-10.0 was 0.051-0.109 and 0.063-0.131 UI/mL, respectively. The cellulolytic activity of G. applanatum and G. tropicum in the temperature range of 35-65 °C was 0.100-0.184 and 0.077-0.110 UI/mL, respectively. Conclusion: The activity of cellulase enzymes were affected by temperature and pH. The cellulolytic activity of G. applanatum was the same as G. tropicum.