Method Development and Validation of Latanoprost by using RP-HPLC in Pharmaceutical Formulations

Abstract

Chromatography was performed with a mobile phase containing a methanol of assay (99.8%) with flow rate of 1ml/min. Quantitation was accomplished with an internal standard method. The procedure was validated for linearity (correlation coefficient = 0.990), accuracy and Limit of detection (LOD) intraday precision. To test validation of the Latanoprost three factors were considered as linearity, precision, LOD where mobile phase, flowrate and pressure are respectively selected as methanol, 1 ml/min, 1600 pascals.