Surface modification of gelatin nanoparticles with polyethylenimine as gene vector

Abstract

A novel carrier on balancing the transfection efficiency and minimizing cytotoxicity was designed. Gelatin cross-linked with 1.8kDa of PEI (GA-PEI 1.8k) formed stable complex and resulted in high positive potential (42.47mV) and buffering effect. These nanoparticles with N/P ratio of 30 give high transfection efficiency 2.12 × 104 RLU/μg protein and cell viability (86.4). These modified GA-PEI nanoparticles, with high transfection efficiency and low cell toxicity, can be a potential gene vector in gene therapy. © 2011 Wei-Ti Kuo et al.