MOLECULAR IDENTIFICATION OF MAIZE (ZEA MAYS L.) GENOTYPES USING START CODON TARGETED (SCoT) MARKERS POLYMORPHISM

Abstract

The genetic diversity estimation in 10 maize (Zea mays L.) genotypes (Somar, Fajer1, Al maha, Baghdad-3, Al hajen nhren, DKC 6777, ZP.glorya, PIOWEE R, KWS, and Syngenta) grown in Iraq was this latest study’s aim. It used DNA markers based on the polymerase chain reaction (PCR). The current study showed the results of molecular detection using the start codon targeted (SCoT) markers to assess the variation of 10 genotypes studied through the existence of single, multi-shapes, and unique packages. Even some primers showed a unique imprint of the genetic structures of maize plants. The synthesis comprised a genetic relationship tree of various genetic structures, constructed to estimate the genetic diversity between maize plants’ different genetic structures. They appeared in varying number and size of multiplier pieces and the efficiency of prefixes used to produce different DNA packages in the maize genome. This research employed DNA markers based on PCR to examine the genetic makeup of these maize cultivars. The study utilized various DNA markers, including those developed by SCoT and others, to examine the genetic variation among the studied maize genotypes. The presented study identified the presence of single, polymorphic, and unique genetic markers, thus, providing a unique fingerprint for specific maize genotypes.