Methods for atomic force microscopy of biological and living specimens

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Abstract

Two main precautions must be taken into account to obtain high-resolution morphological and nanomechanical characterization of biological specimens with an atomic force microscope: the tip-sample interaction and the sample-substrate adhesion. In this chapter we discuss the necessary steps for a correct preparation of three types of biological samples: erythrocytes, bacteria, and osteoblasts. The main goal is to deliver reproducible protocols to produce good cellular adhesion and minimizing the morphological alterations of the specimens.

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Dinarelli, S., Girasole, M., & Longo, G. (2018). Methods for atomic force microscopy of biological and living specimens. In Methods in Molecular Biology (Vol. 1814, pp. 529–539). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8591-3_31

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