Quantitative determination of leptin hormone using gold nanoparticle-based lateral flow assay

Abstract

A lateral flow assay (LFA) has been developed that can be used in point-of-care (PoC) use for the sensitive determination of leptin hormone. The limit of detection value was 0.158 ng/mL and the limit of quantification value was 0.479 ng/mL for leptin hormone. The stability studies showed that the assay response was equal to the initial value even after 6 months. LFA accuracy tests performed in commercial artificial serum samples were compared with the enzyme-linked immunosorbent assay (ELISA). The obtained recovery values between 95 and 110% show that the developed LFA can be used for quantitative determination of leptin hormone. As the result of the study, a fast and disposable assay was developed for the determination of leptin hormone, which can be performed with a small amount of sample in less than 15 min without the need for long-term analysis stages, qualified personnel, expensive equipment and devices.