Enhanced robustness in acetone-butanol-ethanol fermentation with engineered Clostridium beijerinckii overexpressing adhE2 and ctfAB

10Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.

Abstract

Clostridium beijerinckii CC101 was engineered to overexpress aldehyde/alcohol dehydrogenase (adhE2) and CoA-transferase (ctfAB). Solvent production and acid assimilation were compared between the parental and engineered strains expressing only adhE2 (CC101-SV4) and expressing adhE2, ald and ctfAB (CC101-SV6). CC101-SV4 showed an early butanol production from glucose but stopped pre-maturely at a low butanol concentration of ∼6 g/L. Compared to CC101, CC101-SV6 produced more butanol (∼12 g/L) from glucose and was able to re-assimilate more acids, which prevented “acid crash” and increased butanol production, under all conditions studied. CC101-SV6 also showed better ability in using glucose and xylose present in sugarcane bagasse hydrolysate, and produced 9.4 g/L solvents (acetone, butanol and ethanol) compared to only 2.6 g/L by CC101, confirming its robustness and better tolerance to hydrolysate inhibitors. The engineered strain of C. beijerinckii overexpressing adhE2 and ctfAB should have good potential for producing butanol from lignocellulosic biomass hydrolysates.

Cite

CITATION STYLE

APA

Lu, C., Yu, L., Varghese, S., Yu, M., & Yang, S. T. (2017). Enhanced robustness in acetone-butanol-ethanol fermentation with engineered Clostridium beijerinckii overexpressing adhE2 and ctfAB. Bioresource Technology, 243, 1000–1008. https://doi.org/10.1016/j.biortech.2017.07.043

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free