Unliganded thyroid hormone receptor β1 inhibits proliferation of murine fibroblasts by delaying the onset of the G1 cell-cycle signals

Abstract

Thyroid hormone receptors (TRs) are members of the ligand-inducible transcription factor superfamily. The two major functional TRs (α and β1) have different spatial and temporal expression patterns and specific physiological functions for these isoforms are now starting to emerge. By expressing these TR isoforms individually in Swiss 3T3 fibroblasts, we found that TRβ1 expression, in the absence of hormone, provokes a proliferation arrest in G0/G1, lengthening the cycling time. Upon serum stimulation TRβ1-expressing cells showed a marked delay in the induction of cyclins D and E, in the phosphorylation of retinoblastoma protein, and in the activation of cyclin-dependent kinase 2, accompanied by increased levels of cyclin-dependent kinase inhibitor p27Kipl. Accordingly, serum-stimulated E2F-1 transcriptional activity was repressed by TRβ1 in transient transfection experiments. Analysis of the receptor domains required for this effect confirmed that there is no need for a functional ligand-binding domain while the DNA-binding domain is essential. In this work, we demonstrate for the first time that TRβ1 participates in the molecular mechanisms that control cell proliferation. The unliganded TRβ1 impairs the normal induction of the G1/S cycle regulators preventing progression into the S phase.