"Citrus Limetta Extracts Inhibit Oxidative Stress in Human Skin Organ Culture"

Abstract

The current study is part of the development of medicinal plants known for their therapeutic virtues. We were then interested in studying sweet lime (citrus limetta) known for its richness in different compounds characterized by their important biological properties. The present work focused on extracting the peel of sweet lime by the maceration technique. The determination of phenolic compounds (hexane: 2.57 ± 0.0198; ethanol 150 ± 3.4; aqueous: 164.64 ± 2.37 mg equivalent in mg GAE/g DM), and in flavonoids (hexane: 1.544 ± 0.045; ethanol 64.991 ± 1.61; aqueous: 75.915 ± 0.65 mg equivalent mg QE/ g DM). Antioxidant activities were studied by direct, reactive oxygen species assay using chemical systems (TAA, DPPH, ABTS+, FRAP). The antioxidant effect on lipid peroxidation was studied in the biological system (cell culture), by measuring the levels of MDA and DC in HeLa cell line after H 2 O 2 treatment. Our results showed protection against lipid peroxidation, highlighted by a significant decrease in MDA and DC levels (p <0.5 and p <0.01, respectively). The results showed a cytotoxic effect of the hexane extract on HeLa cell lines. In addition, the histological study on the skin fragments showed dose-dependent protection of our extracts against lesions caused by hydrogen peroxide.