Protein profile of the trichloroethylene bacterium capable of degrading

Abstract

A novel bacterial strain, Bacillus cereus 2479 was capable of degrading Trichloroethylene (TCE), grown on TCE containing medium. The total proteome was extracted and profiled through one-dimensional SDS PAGE. TCE has been recognized to be significant environmental pollutants in the soil, ground water and atmosphere. This strain was grown in different minimal medium either with peptone (0.2%) or TCE (0.2%) with peptone. This gel was further examined by densitometer tracing. Nine differentially expressed proteins were found in the bacterium grown in resence of TCE. Out of these nine proteins, three (97.1, 40 and 30 kDa) were found to react immunologically to the antibodies raised against TCE inducible proteins in Western blot analysis. t indicates that some de novo proteins were induced in presence of TCE.