Th9 cells are a subset of CD4+ T cells producing the cytokine, IL-9. Th9 cells are increasingly recognized as being important player in allergy, autoimmunity, and antitumor responses. The polarization and expansion of Th9 cells requires the cytokines IL-4, TGF-β. In this chapter, we described the protocol for measuring Th9 frequencies using whole blood.
Rajamanickam, A., & Babu, S. (2017). Determining the frequencies of th9 cells from whole blood. In Methods in Molecular Biology (Vol. 1585, pp. 83–92). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6877-0_7