Serum 13C-bicarbonate assay for the diagnosis of gastric Helicobacter pylori infection and response to treatment

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Abstract

Background and Aims: Current serological tests for Helicobacter pylori (HP) infection are not useful in assessing active infection or eradication. The feasibility, sensitivity, and specificity of serum 13C-bicarbonate (13C-HCO3) measurement in determining gastric HP before and after eradication by antibiotics were investigated. Methods: Twenty-seven symptomatic patients underwent endoscopy, biopsy, and CLOtest. Patients then consumed a 13C-urea-rich meal; serum was collected before and 1 hour after meal ingestion for quantitative determination of 13C by mass spectrometry. Post-prandial fractional elevation of 13C (Δ13C-HCO3) was correlated with endoscopy, histology, and CLOtest at baseline and at 4 and 8 weeks after therapy. Results: Δ13C-HCO3 ± SEM was 17.02 ± 2.94 in HP-positive patients and 2.77 ± 044 in HP-negative patients (P < 0.0001). In HP-positive patients who responded to therapy, the mean change was initially 20.5 ± 3.1, 3.2 ± 0.91 month after therapy, and 2.8 ± 0.4 2 months after therapy (P < 0.001). The overall sensitivity of this method was 90.6% (95% confidence interval, 74.9-98.0), and its specificity was 85.7% (95% confidence interval, 42.1%-99.6%). Δ13C-HCO3 correlated positively with the degree of histological gastritis and the number of HP organisms. Conclusions: Serum 13C-HCO3 analysis is a novel, simple, and noninvasive method for diagnosis and assessment of eradication of HP infection.

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Kim, M. J., Michener, R., & Triadafilopoulos, G. (1997). Serum 13C-bicarbonate assay for the diagnosis of gastric Helicobacter pylori infection and response to treatment. Gastroenterology, 113(1), 31–37. https://doi.org/10.1016/S0016-5085(97)70077-5

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