Phytochemical profiling and efficacy evaluation of antibacterial potential of Myristica fragrans seed extracts against selected microorganisms

Abstract

Objective: Traditional plants have been proved as a novel source for health-care remedies. The plants are the rich source of bioactive compounds which can be useful for health-care purpose. Emergence of resistance among pathogenic bacteria against available antibiotics is a common phenomenon. Hence, the plant-based bioactive molecule may be an alternative for the development of new formulation. The use of natural bioactive molecules as a parental compound for the development of new formulation may be safer with fewer side effects as compared to synthetic chemical compounds. Myristica fragrans seed is widely used in routine life from ancient time for health care. Traditionally, it is being used in for stomach related complications. Hence, the current study pertained to the phytochemical profiling of M. fragrans seed and its efficacy evaluation against some clinically significant bacterial species. Methods: In this study, ethanol and acetone extract of M. fragrans seed (Jaiphal) were prepared via using soxhlet apparatus. Extract was qualitatively analyzed to check the phytochemical composition. Then antibacterial efficacy of prepared extracts were analyzed by agar well diffusion assay against Escherichia coli, Bacillus subtilis, Bacillus megaterium, and Proteus mirabilis. Results: The result of phytochemical profiling shows the presence of tannin, terpenoids, alkaloids, indole, and phenol. Moreover, M. fragrans seed extract exhibiting significant antibacterial activity against selected micro-organisms. Conclusion: Finding of the study concludes that M. fragrans seed (jiphal) possesses the antimicrobial capabilities against infections and/or diseases caused by chosen micro-organisms and seems to be useful in the discovery of novel antibiotic drugs.