In this study we identify the molecules involved in the MAPK signal transduction pathway (Ras, Raf-1, Mek, Mek-P and MAPK) in highly purified endosomal fractions isolated from rat liver. Biochemical analysis shows that only the early-sorting endocytic compartment contains activated Raf-1 and Mek. Finally, the exogenous administration of EGF led to redistribution of Raf-1 from the caveolin-enriched plasma membrane into the endosomes. Copyright (C) 1998 Federation of European Biochemical Societies.
Pol, A., Calvo, M., & Enrich, C. (1998). Isolated endosomes from quiescent rat liver contain the signal transduction machinery: Differential distribution of activated Raf-1 and Mek in the endocytic compartment. FEBS Letters, 441(1), 34–38. https://doi.org/10.1016/S0014-5793(98)01517-8