Tracheal cartilage regeneration by slow release of basic fibroblast growth factor from a gelatin sponge

33Citations
Citations of this article
13Readers
Mendeley users who have this article in their library.

Abstract

Objective: We investigated whether implantation of a gelatin sponge, releasing basic fibroblast growth factor slowly (b-FGF) into a tracheal cartilage defect, would induce regeneration of autologous tracheal cartilage. Methods: We created a 1-cm defect in the midventral portion of each of 10 consecutive cervical tracheal cartilage rings in 12 experimental dogs. In the control group (n = 4), the resulting defects were left untreated. In the gelatin group (n = 4), empty gelatin sponges were implanted in the defects. In the basic fibroblast growth factor group (n = 4), gelatin sponges incorporating 100 μg of b-FGF solution were implanted in the defects. We killed the 4 dogs in each group at 1, 3, 6, and 12 months after implantation, respectively, and examined the implant sites macro- and microscopically. Results: In the control and gelatin groups, no regenerated cartilage was observed in the tracheal cartilage defects, and the width of the gap between the host cartilage stumps had shrunk. In the b-FGF group, regenerated cartilage was observed in all dogs. The proportion of the defect in the host cartilage occupied by regenerated cartilage was 13%, 84%, 75%, and 69% at 1, 3, 6, and 12 months, respectively. The regenerated cartilage was fibrous cartilage covered with perichondrium, which grew from the host perichondrium and showed continuity with the host cartilage stumps. Conclusions: Implantation of a gelatin sponge slowly releasing basic fibroblast growth factor induces tracheal cartilage regeneration, which subsequently fills a large proportion of experimentally created tracheal cartilage defects within 12 months after implantation. © 2007 The American Association for Thoracic Surgery.

Cite

CITATION STYLE

APA

Igai, H., Yamamoto, Y., Chang, S. S., Yamamoto, M., Tabata, Y., & Yokomise, H. (2007). Tracheal cartilage regeneration by slow release of basic fibroblast growth factor from a gelatin sponge. Journal of Thoracic and Cardiovascular Surgery, 134(1), 170–175. https://doi.org/10.1016/j.jtcvs.2007.02.022

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free