Dehydrocoelenterazine is the organic substance constituting the prosthetic group of Pholasin

Abstract

(Chemical Equation Presented) A little light analysis: Pholasin is composed of dehydrocoelenterazine (DCL), an organic substance from the photoprotein of luminous squid. Treatment of Pholasin with excess dithiothreitol (DTT) produced a solution containing a DCL-DTT adduct, whose structure was determined by LC-MS analysis and compared with the authentic compound. This demonstrated that DCL constituted the prosthetic group of Pholasin. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.