In vitro evaluation of synergistic antimicrobial activity of Trachyspermum ammi and Syzygium aromaticum

Abstract

A remarkable Increase in antibiotic toxicity and multidrug resistance among several group of microorganism are the major challenge worldwide. These new emerging trends led for development of different strategies. Cloves (Syzygium aromaticum) and ajwain (Trachyspermum ammi) are plant species widely used in traditional medicine to treat various infectious diseases. The main objective of this research study is to investigate the antimicrobial effects of acetone extract of the seed of Syzygium aromaticum and Trachyspermum ammi individually and combination of the both (1:1ratio) via disc diffusion method against three bacterial culture of clinical significance like (Staphylococcus aureus, Streptococcus pyrogenes and, Escherichia coli). Both the extract was found active against almost all the bacterial strain tested. Greater sensitivity was observed in Gram positive bacteria as compared to gram negative bacteria. Synergetic effect evaluations demonstrate they have synergetic as well as no antagonist properties. Due to their therapeutic potential they can be used against multidrug resistant microorganisms in combination therapy.