ANTIOXIDANT ACTIVITY AND OPTIMISATION OF ULTRASONIC-ASSISTED EXTRACTION BY RESPONSE SURFACE METHODOLOGY OF ARONIA MELANOCARPA ANTHOCYANINS

Abstract

The Based on one-factor-at-a-time experiments, response surface methodology was employed to optimize ultrasound-assisted extraction of Aronia melanocarpa berries (chokeberry; AMP). The optimum extraction conditions were determined as follows: ethanol concentration 62%, ultrasonic power 198 W, liquid-solid ratio 19 (mL/g) and ultrasonic time 44 min. Under this condition, AMP anthocyanin yield was 4.31893 mg/g with a 1.67 % error compared with the actual value, which showed that the model and the optimization process was feasible. Additionally, superoxide anion radical (O 2-·) radical scavenging activities of AMP extracts and VC was comparatively analyzed and the result revealed that the antioxidant activity of AMP extracts was superior to VC.