A novel capacitance electrochemical method for determination of trace amounts of t-NMP22 by adsorptive FFT continuous fast cyclic voltammetry at Au-Pt Ultramicroelectrode in a flowing solution

Abstract

A novel technique has been developed for the analysis of trace amount of Nuclear Matrix Protein 22 (t-NMP22) using adsorptive fast Fourier transform fast cyclic voltammetry (AFFTFCV) in a flow system. The measurements involved continuously applying a cleaning, stripping potential steps together with a potential ramp onto a gold platinum (Au/Pt) ultramicro disk electrode in a flow system, and measuring a signal based on changes in the electrode capacitance as a result of the adsorption and/or desorption of t-NMP22. The effects of different factors including eluent pH, potential scan rate, and accumulation potential and time were also evaluated. The response was determined to be linear from 5 to 100 pg mL -1 (r 2 = 0.996) and the detection limit was determined to be 1.1 pg mL -1 . The accuracy, precision and sensitivity of the technique make it a promising candidate for the measurement of t-NMP22.