Antiproliferative Effect of Alpha Amyrin on Hep2 Cells by Inducing Cytotoxicity and Oxidant Antioxidant Status Modifications

Abstract

Alpha amyrin, a plant bioactive triterpenoids, present in higher plants and particularly in Pergularia daemia. The compound alpha amyrin is a potent antioxidant, anti-inflammatory and antinociceptive, antipruritic, gastro protective and hepatoprotective agent. The anticancer effect of alpha amyrin has not been examined in human laryngeal cancer model. So this study evaluated its potential on Hep2 cells and anti-oxidant modifications. The current investigations explored the alpha amyrin possessed excellent manner (10, 20, 40, 80,160 and 320) µmol/ml and the IC 50 value was found to be 69.32 µmol/ml. The TBARS level increased and antioxidant levels decreased in alpha amyrin treated Hep2 cells as compare to untreated cancer Hep2 cells. On the basis of our result alpha amyrin possess excellent antiproliferative ability on Hep2 cells by inducing cytotoxicity as well as oxidant antioxidant alterations. Therefore, alpha amyrin has therapeutic potential on various types of cancer.