Optimizing concentrations of growth regulators for in-vitro ginger propagation

Abstract

The study evaluates the performance of ginger in media containing different concentrations of growth regulators. Twenty-eight different treatment combinations of benzylaminopurine (BAP) and naphthalene acetic acid (NAA) incorporated into Murashige and Skoog (MS) medium were evaluated for optimal media composition for ginger micropropagation. The combination of 0.05 mgl -1 NAA and 4.0 mgl -1 BAP gave the highest shoot regeneration rate of 4.25. However, this did not differ significantly (p>0.05) from the result (3.38) from 0.05 mgl -1 NAA and 1.0 mgl -1 BAP. Considering the performance of the shoot tip explants in media and the need to lower the cost of micropropagation, the latter combination (0.05 mgl -1 NAA and 1.0 mgl -1 BAP) with 80 percent explant survival, gave an appropriate concentration of growth regulators in media composition for ginger propagation. This combination also supported root development, and perhaps would eliminate the stage of in vitro rooting.