To test the influence of growth rate on the pattern of fatty acids, Scenedesmus obliquus was exposed, in a chemostat culture, to different dilution rates, as these determine growth rates. Dilution rates varied from 0.0 to 0.48 d-1 (0.0 - 0.15 - 0.33 - 0.45 - 0.48 d-1). In total, only few fatty acids showed a significant correlation to the dilution rate. However, it seemed, that algae from the chemostat with zero flow-through differed more from steady state growing algae (i.e. positive dilution rates) in their patterns of fatty acids than within positive growth rates. Also, carbon and nitrogen were measured and C:N-ratios were calculated. The C:N-ratios support the grouping of the samples into two categories: 'zero dilution rate' and 'positive growth rates at steady state'.
Makulla, A. (2000). Fatty acid composition of Scenedesmus obliquus: Correlation to dilution rates. Limnologica, 30(2), 162–168. https://doi.org/10.1016/S0075-9511(00)80011-0