Back Cover: The Scaffold Design of Trivalent Chelator Heads Dictates Affinity and Stability for Labeling His‐tagged Proteins in vitro and in Cells (Angew. Chem. Int. Ed. 38/2018)

Abstract

Abstract Tris NTA chelators consist of three metal‐chelating N‐nitrilotriacetic acids (NTAs) connected by a scaffold structure. Coupled to a fluorophore or other reporter, tris NTAs can be used as a probe for live‐cell labeling of histidine‐tagged proteins. In their Communication (DOI: 10.1002/anie.201802746), R. Tampé et al. report large differences in affinity and stability between linear, dendritic, and cyclic scaffolds, and clarify which trisNTA scaffold is superior for in vitro and cellular applications.