RSV-induced prostaglandin E2 production occurs via cPLA2 activation: Role in viral replication

0Citations
Citations of this article
18Readers
Mendeley users who have this article in their library.

Abstract

Prostaglandins (PGs) are lipid mediators that participate in the regulation of immunological and inflammatory responses, and PG production can affect viral replication. In this study, we have investigated the mechanism of PGE2 production in airway epithelial cells, following respiratory syncytial virus (RSV) infection, and its role in viral replication. We show that RSV infection strongly induces PGE2 secretion, in a time- and replication-dependent manner, through increased cyclooxygenase-2 (COX-2) expression, which occurs independently from viral or cellular protein synthesis. RSV infection induces arachidonic acid release through induction of cytoplasmic phospholipase A 2 (cPLA2) enzymatic activity and its membrane translocation. Specific inhibitors of cPLA2 significantly block RSV-induced PGE2 secretion, indicating a key role of cPLA2 in viral-induced PG production. Blocking PG secretion, through cPLA2 or COX-2 inhibition, results in impairment of RSV replication and subsequent RSV-mediated epithelial cell responses, suggesting that inhibition of PG secretion could be beneficial in RSV infection by reducing proinflammatory mediator production. © 2005 Elsevier Inc. All rights reserved.

Cite

CITATION STYLE

APA

Liu, T., Zaman, W., Kaphalia, B. S., Ansari, G. A. S., Garofalo, R. P., & Casola, A. (2005). RSV-induced prostaglandin E2 production occurs via cPLA2 activation: Role in viral replication. Virology, 343(1), 12–24. https://doi.org/10.1016/j.virol.2005.08.012

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free