The path of messenger RNA through the ribosome

462Citations
Citations of this article
249Readers
Mendeley users who have this article in their library.

Abstract

Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 Å resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13 to +15, the location of downstream pseudoknots that stimulate -1 translational frame shifting.

Cite

CITATION STYLE

APA

Yusupova, G. Z., Yusupov, M. M., Cate, J. H. D., & Noller, H. F. (2001). The path of messenger RNA through the ribosome. Cell, 106(2), 233–241. https://doi.org/10.1016/S0092-8674(01)00435-4

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free