Genetic variation and relationships of Zea mays and Sorghum species using RAPD-PCR and SDS-PAGE of seed proteins

Abstract

Genetic relationship between some species of Zea mays and Sorghum was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of seed protein and random amplification of polymorphic DNA (RAPD-PCR) markers. According to SDS-PAGE analysis, 78 band were identified across the studied species. The number of bands varies from 17 bands in sample number 5 to 6 in sample number 6. Analysis of RAPD-PCR of DNA provided more precise information concerning relationships between Zea mays and Sorghum species than SDS-PAGE analysis. A remarkable result from this study was identifying a close relationship between Zea mays spp mays and Zea mays spp Mexicana. Further support comes from the molecular data of RAPD, which indicate that close relationship between Sorghum valgare and Sorghum bicolor.