Phenomic and physiological analysis of salinity effects on lettuce

Abstract

Salinity is a rising concern in many lettuce-growing regions. Lettuce (Lactuca sativa L.) is sensitive to salinity, which reduces plant biomass, and causes leaf burn and early senescence. We sought to identify physiological traits important in salt tolerance that allows lettuce adaptation to high salinity while maintaining its productivity. Based on previous salinity tolerance studies, one sensitive and one tolerant genotype each was selected from crisphead, butterhead, and romaine, as well as leaf types of cultivated lettuce and its wild relative, L. serriola L. Physiological parameters were measured four weeks after transplanting two-day old seedlings into 350 mL volume pots filled with sand, hydrated with Hoagland nutrient solution and grown in a growth chamber. Salinity treatment consisted of gradually increasing concentrations of NaCl and CaCl2 from 0 mM/0 mM at the time of transplanting, to 30 mM/15 mM at the beginning of week three, and maintaining it until harvest. Across the 10 genotypes, leaf area and fresh weight decreased 0–64% and 16–67%, respectively, under salinity compared to the control. Salinity stress increased the chlorophyll index by 4–26% in the cultivated genotypes, while decreasing it by 5–14% in the two wild accessions. Tolerant lines less affected by elevated salinity were characterized by high values of the chlorophyll fluorescence parameters Fv/Fm and instantaneous photosystem II quantum yield (QY), and lower leaf transpiration.