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A complete RNA-Seq analysis involves the use of several different tools, with substantial software and computational requirements. The Galaxy platform simplifies the execution of such bioinformatics analyses by embedding the needed tools in its web interface, while also providing reproducibility. Here, we describe how to perform a reference-based RNA-Seq analysis using Galaxy, from data upload to visualization and functional enrichment analysis of differentially expressed genes.
Batut, B., van den Beek, M., Doyle, M. A., & Soranzo, N. (2021). RNA-Seq Data Analysis in Galaxy. In Methods in Molecular Biology (Vol. 2284, pp. 367–392). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1307-8_20