Light-addressable potentiometric sensor (LAPS) combined with magnetic beads for pharmaceutical screening

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Abstract

The light-addressable potentiometric sensor (LAPS) has the unique feature to address different regions of a sensor surface without the need of complex structures. Measurements at different locations on the sensor surface can be performed in a common analyte solution, which distinctly simplifies the fluidic set-up. However, the measurement in a single analyte chamber prevents the application of different drugs or different concentrations of a drug to each measurement spot at the same time as in the case of multi-reservoir-based set-ups. In this work, the authors designed a LAPS-based set-up for cell culture screening that utilises magnetic beads loaded with the endotoxin (lipopolysaccharides, LPS), to generate a spatially distributed gradient of analyte concentration. Different external magnetic fields can be adjusted to move the magnetic beads loaded with a specific drug within the measurement cell. By recording the metabolic activities of a cell layer cultured on top of the LAPS surface, this work shows the possibility to apply different concentrations of a sample along the LAPS measurement spots within a common analyte solution.

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Wagner, T., Vornholt, W., Werner, C. F., Yoshinobu, T., Miyamoto, K. ichiro, Keusgen, M., & Schöning, M. J. (2016). Light-addressable potentiometric sensor (LAPS) combined with magnetic beads for pharmaceutical screening. Physics in Medicine, 1, 2–7. https://doi.org/10.1016/j.phmed.2016.03.001

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