Action of the natural compound gomisin a on Ca 2+ movement in human prostate cancer cells

Abstract

Gomisin A is a dietary lignan compound isolated from the fruit of Schisandra chinensis and has many pharmacological properties, including hepato-protective, anti-diabetic, and anti-oxidative activities. However, the benefit of gomisin A is still not well understood. The action of gomisin A is diverse. However, the effect of gomisin A on Ca2+signaling in prostate cancer cells is unknown. Ca2+is a pivotal second envoy that triggers and regulates cellular processes such as apoptosis, fertilization, energy transduction, secretion, and protein activation. The goal of this study was to explore the action of gomisin A on [Ca2+]iand cytotoxicity in PC3 prostate cancer cells. Gomisin A at 100-200 μM provoked [Ca2+]iraises. 20% of the response was reduced by removing external Ca2+. The Ca2+influx provoked by gomisin A was suppressed by 20% by store-caused Ca2+entry suppressors: econazole, SKF96365, nifedipine; also by phorbol 12-myristate 13 acetate and GF109203X. Without external Ca2+, gomisin A-caused [Ca2+]iraises were abolished by thapsigargin. In contrast, gomisin A suppressed the [Ca2+]iraises caused by thapsigargin. U73122 fell short to change gomisin A-caused [Ca2+]iresponses. Gomisin A (20-100 μM) elicited cytotoxicity in a dose-associated fashion. Blockade of [Ca2+] elevations with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid/acetoxy methyl failed to inhibit cytotoxicity of gomisin A. Collectively, gomisin A evoked [Ca2+]iraises and provoked cytotoxicity in a Ca2+-dissociated fashion in prostate cancer cells.