Further Insights into Influence of Light Intensities on the Production of Long-Chain Hydroxy Fatty Acids, Fatty Diols and Fatty Alcohols in Nannochloropsis oceanica

Abstract

Microalgae can modify their metabolic pathways as a response to environmental stimuli such as light, temperature, salinity, and nutrient availability, which critically influence the synthesis of lipids and other biomolecules. While extensive studies have focused on the impact of these environmental variables on the accumulation of valuable compounds such as polyunsaturated fatty acids (PUFAs) and triacylglycerols (TAGs), information on the biosynthesis of specialized metabolites, including long-chain hydroxy fatty acids (LCHFAs), long-chain diols (LCDs), and long-chain alkenols (LCAs) is scarce. These metabolites are thought to contribute to the structural integrity of cell walls in certain microalgae, such as Nannochloropsis spp. (Eustigmatophyceae), where they make up a biopolymer known as algaenan. This study investigates how varying light intensities affect the production of LCHFAs, LCDs, and LCAs in Nannochloropsis oceanica over a 12 h light/dark cycle. Our findings provide insights into the lipid biosynthetic pathways in microalgae, revealing that light strongly drives the production of LCHFAs, whereas LCDs and LCAs are less light-dependent and show more variable responses to different light intensities.