Long stretches of “rare” codons are known to severely inhibit the efficiency of translation. Understanding the distribution of such rare codons is<br />of critical importance in improving the efficiency of heterologous gene expression systems. Accurate estimates of codon usage take the<br />abundance of each protein into consideration. In this paper, we analyze the correlation between approximate measures of codon usage and the<br />availability of tRNA at various growth rates in E coli. We show that the computationally derived estimates of tRNA isoacceptor concentration<br />enable the finding of poorly translated codons.
Ponnala, L. (2009). On finding poorly translated codons based on their usage frequency. Bioinformation, 4(2), 63–65. https://doi.org/10.6026/97320630004063