[...]the PCR amplicons were purified with Performa DTR Gel Filtration Cartridges (Edge Bio, United States of America) and sequenced by an ABI Genetic Analyzer model 3130XL (Life Technologies, Japan). Furthermore, a recent study reported that 63% (28/44) of long-tailed macaques in Laos were infected with P. cynomolgi, which can also be infectious to humans [8,10]. [...]an investigation of human infection with P. cynomolgi is also needed in Laos. [...]previous studies demonstrated that false-positive results for P. vivax and P. falciparum have been observed for P. knowlesi mono-infection with certain RDTs [11,12]. First case of a naturally acquired human infection with Plasmodium cynomolgi.
Iwagami, M., Nakatsu, M., Khattignavong, P., Soundala, P., Lorphachan, L., Keomalaphet, S., … Kano, S. (2018). First case of human infection with Plasmodium knowlesi in Laos. PLOS Neglected Tropical Diseases, 12(3), e0006244. https://doi.org/10.1371/journal.pntd.0006244