First evidence of sucrose biosynthesis by single cyanobacterial bimodular proteins

9Citations
Citations of this article
23Readers
Mendeley users who have this article in their library.

Abstract

The net synthesis of sucrose (Suc) is catalysed by the sequential action of Suc-phosphate synthase (SPS) and Suc-phosphate phosphatase (SPP). SPS and SPP from Anabaena sp. PCC 7120 (7120-SPS and 7120-SPP) define minimal catalytic units. Bidomainal SPSs, where both units are fused, occur in plants and cyanobacteria, but they display only SPS activity. Using recombinant proteins that have fused 7120-SPS and 7120-SPP, we demonstrated that they are bifunctional chimeras and that the arrangement 7120-SPS/SPP is the most efficient to catalyse the sequential reactions to yield Suc. Moreover, we present the first evidence of a bidomainal SPS present in the cyanobacterium Synechococcus elongatus PCC 7942 with both, SPS and SPP activity. © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Cite

CITATION STYLE

APA

Martínez-Noël, G. M. A., Cumino, A. C., De Los Angeles Kolman, M., & Salerno, G. L. (2013). First evidence of sucrose biosynthesis by single cyanobacterial bimodular proteins. FEBS Letters, 587(11), 1669–1674. https://doi.org/10.1016/j.febslet.2013.04.012

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free