Bio-load of Mycobacterium avium subspecies paratuberculosis was estimated in the first mass screening of human population in Mathura region of South Uttar Pradesh. Of the 48,919 samples collected between December, 2010 and March, 2013 from Pathology laboratories, 26,390 were screened by indigenous ELISA kit, IS900 blood and stool PCR, IS 1311 PCR_REA and stool microscopy. Of the 23,196 serum samples screened by indigenous ELISA, 34.0% were positive for MAP infection (Mathura-35.4% and Agra 14.2%). Percent prevalence of MAP infection was 28.3, 41.8, 37.4, 29.5, 41.1, 40.7, 42.5, 36.5 and 51.2 in patients suspected for diabetes, liver disorders, anaemia, thyroid disorder, tuberculosis, typhoid, abdominal disorders, inflammatory illness and ion imbalance, respectively. Of 3093 blood samples screened by IS900 PCR, 8.4%) were positive (Mathura-9.2% and Agra-7.9%). Percent prevalence of MAP was 4.8, 7.0, 20.0, 4.9, 17.8, 7.6 and 12.7 in patients suspected for diabetic, liver disorder, skin disorders, anaemia, Malaria, typhoid and apparently normal individuals, respectively. Of the 101 stool samples screened by microscopy, 5.9% were positive and of these 2.9% were confirmed by IS900 PCR. IS7371 PCR_REA bio-typing showed 'Indian Bison Type' was the most prevalent biotype. Study indicated large scale exposure of human population to MAP infection in the Mathura region of South Uttar Pradesh and like in animals'Indian Bison Type' was the most prevalent biotype of MAP infecting human beings in this region. © 2014 Asian Network for Scientific Information.
S.V., S., N., K., J.S., S., A.V., S., P.K., S., N.D., A., … R., D. (2014). First mass screening of the human population to estimate the bio-load of mycobacterium avium subspecies paratuberculosis in north India. Journal of Biological Sciences. S. V. Singh, Microbiology Laboratory, Central Institute for Research on Goats (CIRG), Makhdoom, PO-Farah, Mathura-281122, Uttar Pradesh, India: Asian Network for Scientific Information (308-Lasani Town, Sargodha Road, Faisalabad, Pakistan). Retrieved from http://scialert.net/qredirect.php?doi=jbs.2014.237.247&linkid=pdf