Functional characterization of the ER stress induced X-box-binding protein-1 (Xbp-1) in the porcine system

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Abstract

The unfolded protein response (UPR) is an evolutionary conserved adaptive reaction for increasing cell survival under endoplasmic reticulum (ER) stress conditions. X-box-binding protein-1 (Xbp1) is a key transcription factor of UPR that activates genes involved in protein folding, secretion, and degradation to restore ER function. The UPR induced by ER stress was extensively studied in diseases linked to protein misfolding and aggregations. However, in the porcine system, genes in the UPR pathway were not investigated. In this study, we isolated and characterized the porcine Xbp1 (pXbp1) gene in ER stress using porcine embryonic fibroblast (PEF) cells and porcine organs. ER stress was induced by the treatment of tunicamycin and cell viability was investigated by the MTT assay. For cloning and analyzing the expression pattern of pXbp1, RT-PCR analysis and Western blot were used. Knock-down of pXbp1 was performed by the siRNA-mediated gene silencing.

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Zhang, J. Y., Lee, K. S., Kim, J. S., Song, B. S., Jin, D. I., Koo, D. B., & Yu, K. (2011). Functional characterization of the ER stress induced X-box-binding protein-1 (Xbp-1) in the porcine system. BMC Molecular Biology, 12. https://doi.org/10.1186/1471-2199-12-25

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