Functionally rodless mice: Transgenic models for the investigation of cone function in retinal disease and therapy

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Abstract

Two genetically engineered strains of mice were used to characterize murine cone function electroretinographically, without interference of rod-driven responses: (1) mice with a deletion of the gene for the rod transducin α-subunit (transducin α-/-), and (2) mice with rod arrestin deleted (arrestin -/-). In the first three months of age, both strains have a normal complement of rods and normal rod structure, but transducin α-/- mice have no rod-driven responses to light, while rod-driven activity of arrestin -/- mice can be suppressed by a single intense flash for hours. In response to intense flashes the electroretinograms of these strains of mice showed a readily identifiable, pure-cone a-wave of ∼10 μV saturating amplitude. A 530 nm background that saturates rod responses of wild type mice was found to desensitize the b-wave responses of mice of both transgenic lines, whether the b-waves were driven by photons captured by M- or UV-cone pigments. The desensitizing effect of the 530 nm background on UV-pigment driven responses provides new evidence in support of the hypothesis of functional co-expression of the M-pigment in cones expressing primarily the UV-pigment. © 2002 Published by Elsevier Science Ltd.

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Lyubarsky, A. L., Lem, J., Chen, J., Falsini, B., Iannaccone, A., & Pugh, E. N. (2002). Functionally rodless mice: Transgenic models for the investigation of cone function in retinal disease and therapy. Vision Research, 42(4), 401–415. https://doi.org/10.1016/S0042-6989(01)00214-0

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