The Gα protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gβ subunits

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Abstract

G protein coupled receptors (GPCR) sense diverse ligands and signal via heterotrimeric G proteins. The Saccharomyces cerevisiae GPCR Gpr1 senses glucose and controls filamentous growth via an unusual Gα protein, Gpa2, which lacks any known Gβγ subunits. Our genetic and biochemical studies identify Gpa2 interaction partners (Gpb1/2, Gpg1) and provide evidence that these proteins function as G protein subunit mimics and signaling effectors. Gpb1 and Gpb2 lack the seven WD-40 repeats found in Gβ subunits and instead contain seven kelch repeats implicated in protein-protein interactions. Gβ subunits and the kelch repeat protein galactose oxidase fold into strikingly similar seven-bladed β propellers. Our studies demonstrate that Gpa2 signals in conjunction with Gβ structural mimics and that homologous G protein subunits or effectors may be conserved in multicellular eukaryotes.

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Harashima, T., & Heitman, J. (2002). The Gα protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gβ subunits. Molecular Cell, 10(1), 163–173. https://doi.org/10.1016/S1097-2765(02)00569-5

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