The physiological function of the type 1 NAD(P)H dehydrogenase (Ndh-1) of Synechocystis sp. PCC6803 has been investigated by inactivating the gene ndhH encoding a subunit of the complex. Molecular analysis of independent transformants revealed that all clones were heteroploid, containing both wild-type and mutant ndhH copies, whatever the metabolic conditions used during genome segregation, including high CO2 concentration. By replacing the chromosomal copy of the ndhH gene by a plasmidial copy under the control of a temperature-controlled promoter, we induce a conditional phenotype, growth being only possible at high temperature. This clearly shows for the first time that an ndh gene is indispensable to the survival of Synechocystis sp. PCC6803. © 2000 Federation of European Biochemical Societies.
Pieulle, L., Guedeney, G., Cassier-Chauvat, C., Jeanjean, R., Chauvat, F., & Peltier, G. (2000). The gene encoding the NdhH subunit of type 1 NAD(P)H dehydrogenase is essential to survival of Synechocystis PCC6803. FEBS Letters, 487(2), 272–276. https://doi.org/10.1016/S0014-5793(00)02369-3