Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene

0Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.

Abstract

Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing, karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.

Cite

CITATION STYLE

APA

Khudiakov, A., Kostina, D., Zlotina, A., Nikulina, T., Sergushichev, A., Gudkova, A., … Kostareva, A. (2017). Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene. Stem Cell Research, 24, 77–80. https://doi.org/10.1016/j.scr.2017.08.015

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free