Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts

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Abstract

Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.

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Questa, M., Romorini, L., Blüguermann, C., Solari, C. M., Neiman, G., Luzzani, C., … Miriuka, S. G. (2016). Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts. Stem Cell Research, 16(2), 300–303. https://doi.org/10.1016/j.scr.2015.12.026

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