Genome size and identification of abundant repetitive sequences in Vallisneria spinulosa

  • Feng R
  • Wang X
  • Tao M
  • et al.
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Abstract

Vallisneria spinulosa is a freshwater aquatic plant of ecological and economic importance. However, there is limited cytogenetic and genomics information on Vallisneria . In this study, we measured the nuclear DNA content of Vallisneria spinulosa by flow cytometry, performed a de novo assembly, and annotated repetitive sequences by using a combination of next-generation sequencing (NGS) and bioinformatics tools. The genome size of Vallisneria spinulosa is approximately 3,595 Mbp, in which nearly 60% of the genome consists of repetitive sequences. The majority of the repetitive sequences are LTR-retrotransposons comprising 43% of the genome. Although the amount of sequencing data used in this study was not sufficient for a whole-genome assembly, it could generate an overview of representative elements in the genome. These results will lay a new foundation for further studies on various species that belong to the Vallisneria genus.

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Feng, R., Wang, X., Tao, M., Du, G., & Wang, Q. (2017). Genome size and identification of abundant repetitive sequences in Vallisneria spinulosa . PeerJ, 5, e3982. https://doi.org/10.7717/peerj.3982

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