The dynamics of changes in membrane potential, cytosolic free Ca2+ [Ca2+]i and immunoreactive insulin release were assessed in RINm5F cells. Membrane depolarization and a rise in [Ca2+]i preceded the stimulation of insulin release by D-glyceraldehyde. Forskolin, which raised the cellular cyclic AMP levels, stimulated insulin release without changing membrane potential or [Ca2+]i. It is concluded that cyclic AMP acts on insulin release not by mobilizing Ca2+ but by another, as yet undefined, mechanism. © 1984.
Wollheim, C. B., Ullrich, S., & Pozzan, T. (1984). Glyceraldehyde, but not cyclic AMP-stimulated insulin release is preceded by a rise in cytosolic free Ca2+. FEBS Letters, 177(1), 17–22. https://doi.org/10.1016/0014-5793(84)80972-2