Gold Nanobeacons for Tracking Gene Silencing in Zebrafish

  • Cordeiro M
  • Carvalho L
  • Silva J
  • et al.
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Abstract

© 2017 by the authors; licensee MDPI, Basel, Switzerland.The use of gold nanoparticles for effective gene silencing has demonstrated its potential as a tool for gene expression experiments and for the treatment of several diseases. Here, we used a gold nanobeacon designed to specifically silence the enhanced green fluorescence protein (EGFP) mRNA in embryos of a fli-EGFP transgenic zebrafish line, while simultaneously allowing the tracking and localization of the silencing events via the beacon’s emission. Fluorescence imaging measurements demonstrated a decrease of the EGFP emission with a concomitant increase in the fluorescence of the Au-nanobeacon. Furthermore, microinjection of the Au-nanobeacon led to a negligible difference in mortality and malformations in comparison to the free oligonucleotide, indicating that this system is a biocompatible platform for the administration of gene silencing moieties. Together, these data illustrate the potential of Au-nanobeacons as tools for in vivo zebrafish gene modulation with low toxicity which may be used towards any gene of interest.

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Cordeiro, M., Carvalho, L., Silva, J., Saúde, L., Fernandes, A., & Baptista, P. (2017). Gold Nanobeacons for Tracking Gene Silencing in Zebrafish. Nanomaterials, 7(1), 10. https://doi.org/10.3390/nano7010010

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