Objectives: The main effect of HMB is to increase lean body mass and muscle strength, the main function of glutamine is to resist fatigue. In order to explore the effects of Mice' capacities on aerobic exercise when united supplements HMB and Glu or only HMB and Glu. Then explore whether united suppliants have collaborative effects, and provide theory of reference on nutrition supplements. Methods: We choose 40 clean and healthy Kunming mice, and divide them 10 a group. After 2 weeks'adaptability feed, the mice are randomly divided into control group (normal diet), sport + HMB group (0.45 g/kg body weight HMB lavage daily), sport + glutamine group (2.7 g/kg body weight glutamine lavage daily), sport + HMB + glutamine group (0.45 g/kg body weight HMB and 2.7 g/kg body weight glutamine lavage daily). All the mice weighed once a week to determine drug doses. In the study we set animal mice model by swimming. After adaptability feed, all the mice swim 40min without weight loading two days a time(water temperature is 19°C, water depth is 15 cm, the pool diameter is 40 cm), in the second week swim 70min two days a time, in the third week swim 100 min and swim 110 min in the fourth week. In the mice swimming process, we use sticks to stir to ensure the physiological load of exercise, after that record the swimming time. We pick eyeball to take 1ML blood, and then kill mice by dislocate the neck after swimming immediately, the blood is used to test routine blood and BUN level. Put the mice which are taken blood on the stage to take the double leg calf muscles, liver, and the determination of the muscle of mice malondialdehyde (MDA), superoxide dismutase (SOD), liver glycogen, and succinate dehydrogenase (SDH), blood BUN and other biochemical indicators. Use the single factor analysis of variance to compare HMB and glutamine supplement and single irrigation suits HMB, glutamine differences in ability of aerobic exercise in mice. Results: After supplying four weeks, the results indicate that the mixed group's weight, the swimming time of exhaustion, MDA, SOD, SDH, hepatic glycogen and BUN all have significant differences when comparing with the other groups (P<0.01). Meanwhile, the mixed group's content of MDA in gastrocnemius and BUN in blood are the lest, and the content of SOD, SDH and hepatic glycogen are the most. Conclusions: (1) Supplementary feeding glutamine alone, HMB and glutamine are combined supplementary feeding can all significantly increase the weight of mice, while the HMB group' weight were less than the control group, which may indicate that the effect of increasing weight is not obvious. (2) HMB have the best effect of prolonging the swimming time ,which may explain the relatively light weight, aerobic capacity is relatively strong. (3) HMB, Glu supplementary feeding mice either alone or combined complementary, which all increase the SOD and reduce the MDA, indicating that nutritional supplements can improve the capacity of Anti-fatigue oxidationcapacity, and the joint feeding is the most obvious. (4) HMB, Glu supplementary feeding mice either alone or combined, which all can significantly increase the glycogen content in mice. Meanwhile, the effect of HMB alone and HMB, glutamine jointly feeding are quite similar. (5) HMB, Glu supplementary feeding mice either alone or combined, which all can significantly increase the content of SDH. Meanwhile, the joint feeding have the more obvious effect.
Yuanxun, H., Youbin, S., Xiaofei, H., Huang, L., Qilong, W., & Yuanyuan, L. (2014). GW25-e5329 Research on the Effects of Exhaustive Swimming Mice’ cardio-pulmonary function after united Supplements of HMB and Glutamine. Journal of the American College of Cardiology, 64(16), C212. https://doi.org/10.1016/j.jacc.2014.06.989