HAIRY ROOT CULTURE OF AN IMPORTANT MEDICINAL PLANT: COLEUS FORSKOHLII

  • R U
  • C S
  • et al.
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Abstract

An expt. was conducted for induction & establishment of Agrobacterium mediated hairy root culture of Coleus forskohlii was developed & co-cultivation on hormone free semisolid {MS} Medium with B5 vitamins. Sterilization period was standardized for the stem & leaf explant using 0.1% bavistin and 0.1% Hgcl2 with varied intervals It resulted in the emergence of hairy roots from the leaf explant, stem explant after 20th day of infection. The transformation of hairy roots was established and confirmation of forskolin content was done by {HPLC}. Selection of high forskolin can provide an alternative source of large scale prodn. of forskolin. [on {SciFinder}(R)]

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R, U. M., C, S., J, J., & A, L. P. (2014). HAIRY ROOT CULTURE OF AN IMPORTANT MEDICINAL PLANT: COLEUS FORSKOHLII. International Journal of Agriculture Sciences, 3(2), 82–89. https://doi.org/10.9735/0975-3710.3.2.82-89

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