High-performance liquid-liquid chromatography

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Abstract

The applicability of liquid-liquid systems as phase systems in high-performance liquid chromatography (HPLC) was investigated. The more polar phase of the liquid-liquid system is generated in situ on pre-packed silica columns by pumping through the saturated mobile phase. The influence of the type of silica support on the amount of generated stationary liquid, the phase ratio, the retention and the selectivity was measured. The column efficiency and capacity of liquid-liquid systems generated in situ were compared with normal adsorption systems. The results obtained so far indicate that liquid-liquid systems can be a worthwhile way to increase the number of phase systems in HPLC. Their practical utility is demonstrated with a number of separations of different classes of compounds. © 1984.

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Crombeen, J. P., Heemstra, S., & Kraak, J. C. (1984). High-performance liquid-liquid chromatography. Journal of Chromatography A, 286(C), 119–129. https://doi.org/10.1016/S0021-9673(01)99179-4

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