A high-throughput screen for MscL channel activity and mutational phenotyping

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Abstract

A novel fluorescence-based screen for bacterial mechanosensitive ion-channel activity has been developed. This assay is capable of clearly distinguishing the previously observed gain of function and loss of function phenotypes for the Escherichia coli mechanosensitive channel of large conductance (Ec-MscL). The method modifies Molecular Probes' Live/Dead® BacLight™ bacterial viability assay to monitor MscL channel activity as a function of bacterial survival from osmotic downshock. © 2001 Published by Elsevier Science B.V.

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Maurer, J. A., & Dougherty, D. A. (2001). A high-throughput screen for MscL channel activity and mutational phenotyping. Biochimica et Biophysica Acta - Biomembranes, 1514(2), 165–169. https://doi.org/10.1016/S0005-2736(01)00390-X

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