A typical hydrophilic enzyme, CT, can be dissolved in nonpolar organic solvents (n-octane, cyclohexane and toluene) up to μM concentrations. In the homogeneous solution obtained, the enzyme possesses catalytic activity and enormously high thermostability. It does not lose this activity even after several hours refluxing in octane (126°C) or cyclohexane (81°C). © 1991.
Mozhaev, V. V., Poltevsky, K. G., Slepnev, V. I., Badun, G. A., & Levashov, A. V. (1991). Homogeneous solutions of hydrophilic enzymes in nonpolar organic solvents New systems for fundamental studies and biocatalytic transformations. FEBS Letters, 292(1–2), 159–161. https://doi.org/10.1016/0014-5793(91)80857-Y